Development of suspension culture from callus. Production of secondary metabolites. Artificial seed production. Shake flask studies; comparison of yield in synthetic and complex media, Fed batch culture. Preparation of Fermentor, ethanol production in Fermentor- study of growth, product formation kinetics. Single cell protein (SCP) production by continuous culture.

1. Callus Induction Techniques
2. Development of suspension culture from callus
3. Production of Secondary metabolite-Anthocyanin
4. Artificial seed production (Axillary buds)
5. Shake flask studies:Comparison of yield in synthetic and complex
6. Estimation of DNA (By diphenyl method)
7. Estimation of unknown protein by Lowry’s method 
8. Fed batch culture –Assessment of yield
9. Development of inocula;lag time effect
10. Preparation of the fermenter
11. Production of Ethanol in fermenter-Study of growth, product formation kinetics, end substrate utilization
12. Single Cell Protein(SCP) production by continuous culture