Separation methods for various Bioprocesses; Sedimentation, centrifugation, filtration. Product enrichment operations: precipitation – protein fractionation, two-phase aqueous extraction. Separation of amino acids/Carbohydrates by TLC Dot blots techniques for protein characterization. Estimation of ethanol and citric acid from fermented broth.

1. Cell disruption techniques
2. Solid-liquid separation methods: Filtration
3. Solid-liquid separation methods: Sedimentation
4. Solid-liquid separation methods: Centrifugation
5. Product enrichment operations: Precipitation – (NH4)2SO4 fractionation of a protein
6. Product enrichment operations: Two phase aqueous extraction
7. Product drying techniques
8. Separation of Amino acids/Carbohydrates by TLC
9. Characterization of protein by Western blotting
10.Characterization of protein by Dot blot
11. Estimation of Citric acid from fermented broth
12. Estimation of Ethanol from fermented broth